PDGF와 LPS가 치주 인대 세포의 활성에 미치는 영향에 관한 연구
The Effects of PDGF and LPS on the Viabillty of Human Periodontal Ligament Cells
허정, 임정현, 최종범,
소속 상세정보
허정 ( ) - 원광대학교 치과대학 교정학교실
임정현 ( ) - 원광대학교 치과대학 교정학교실
최종범 ( ) - 원광대학교 치과대학 교정학교실
KMID : 0361919980280010143
Abstract
Platelet-derived growth factor(PDGF) and lipopolysaccharide(LPS) may be the important regualtors of bone metabolism. Exogenous PDGF is recognized to have a stimulating effect on bone resorption in organ culture, but to stimulate the formation of new bone ultimately. LPS is known to be a stimulating agent on the osteoclastic activity.
The purpose of this study was to evaluate the effects and the interaction of PDGF and LPS on periodontal ligament(PDL) cells which have important roles in bone remodeling.
Cultured human periodontal ligament cells were treated with various concentration of PDGF and/or LPS. The cellular viability was measured by Microtitration(MTT) assay according to the lapse time of culture.
The obtained results were as follows:
1. The viability of PDL cells was not different from the control in O.lng/nil of PDGF, but was significantly increased to be over the level of control in lng/ml of PDGF at the second day of culture, and in lOng/ml of PDGF at the second and the third day of culture.
2. The cellular viability was decreased in O.5uglnil or 5u8/ml of LPS at the third day of culture.
3. Incubation with both 1ng/ml or 10ng/rnl of PDGF and U.5AOml or 5pg/ml of LPS resulted in the increased cellular viability at the third day, which. was greater than LPS only treated group. It was greater than even the control group in lOng/ml of PDGF.
From the above findings, we could summarize that the admixture of PDGF and LPS could not less increase the viability of the human periodontal ligament cells than PDGF only.
키워드
PDGF;LPS;골 개조;PDGF;LPS;BONE REMODELING
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